Description : |
Activin-A is a TGF-beta family member that exhibits a wide range
of biological activities including regulation of cellular proliferation
and differentiation, and promotion of neuronal survival. Elevated levels
of Activin-A in human colorectal tumors and in post-menopausal woman have
been implicated in colorectal and breast cancers, respectively.
The biological activities of Activin-A can be neutralized by inhibins
and by the diffusible TGF-beta antagonist, Follistatin. Human Activin-A is a
26 kDa disulfide-linked homodimer of two beta A chains, each containing
116 amino acid residues.
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Catalog #: |
003-ACTIVINa |
Source : |
High-5 (BTI-Tn-5B1-4) Insect Cells |
Formulation : |
The sterile filtered solution was lyophilized from 50 mM Citric Acid, pH 3.0. |
Stability : |
The lyophilized protein is stable for a few weeks at room temperature,
but best stored at -800C.
Reconstituted Human Activin-A should be stored in
working aliquots at -800C. |
Purity : |
Greater than 95% by SDS-PAGE and HPLC analysis.
Endotoxin level is less than 0.1 ng per µg (1EU/µg). |
Reconstitution : |
We recommend a quick spin followed by reconstitution in
water to a concentration of 50 µg/ml (e.g., 5 µg/100µl) will yield a solution of
10 mM Citric Acid, pH 3.0. This solution can then be stored at room temperature or
at 40C for 1 month. It is recommended that further dilutions be made in PBS
containing 2 mg/ml albumin and stored at -800C.
|
Biological Activity : |
The ED50 was determined by ability to inhibit
the murine MPC-11 cells. The expected ED50 is 0.5 - 2.0 ng/ml.
|
PubMed Link : |
Activin-A Human |
Usage: |
For research Purposes Only - Made using a cell BTI-Tn-5B1-4 under license from the Boyce Thompson Institute for Plant Research, Inc. Not for use in diagnostic or therapeutic procedures. |
Country of Origin : |
USA |
Experience - Innovation - Persistence
