Recombinant Human Soluble Fas Ligand (FasL)

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FasL Applications

Description :

FasL is a type II membrane protein belonging to the TNF family of cytokines. FasL induces apoptosis when it binds to cells expressing the Fas antigen. FasL was first discovered on the cell surface of T cells and NK cells. Expressed on the cell surface of activated T cells, FasL kills T cells and activated B cells leading to down-regulation of the immune responce. The full length of FasL contains 281 amino acid residues, consisting of the transmembrane domain, extracellular domain and the cytoplasmic region. A metalloproteinase cleaves the membrane-bound FasL to produce soluble FasL (sFasL), which exists as a homotrimer. The sFasL shows lower cytotoxic activity than membrane bound FasL. SBH Sciences's recombinant Fas Ligand is a soluble 19.9 kDa protein containing 175 amino acid residues, comprising of only the extracellular domain of FasL.

Catalog #:

001-HFASL

Source :

CHO cells

Formulation :

The sterile filtered solution was lyophilized from 1 x PBS, pH 7.2

Stability :

The lyophilized protein is stable for a few weeks at room temperature, but best stored at -20⁰C. Reconstituted sFasL should be stored in working aliquots at -20⁰C.

Purity :

Greater than 98% by SDS-PAGE. Endotoxin level is less than 0.1 ng per 痢 (1EU/痢).

Reconstitution :

We recommend a quick spin followed by reconstitution in water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other buffers and stored at 4⁰C for 1 week or stored at -20⁰C for future use.

Biological Activity :

Determined by it's ability to induce cytotoxicity in Jurkat cells in the absence of any cross-linking. The ED₅₀ for this effect is 2-4 ng/ml, corresponding to a specific activity of > 1 x 10⁵ units/mg.

AA Sequence :

LQKELAELRE STSQMHTASS LEKQIGHPSP PPEKKELRKV AHLTGKSNSR SMPLEWEDTY GIVLLSGVKY KKGGLVINET GLYFVYSKVY FRGQSCNNLP LSHKVYMRNS KYPQDLVMME GKMMSYCTTG QMWARSSYLG AVFNLTSADH LYVNVSELSL VNFEESQTFF GLYKL

PubMed Link :

Fas Ligand

Usage:

For research only. Not for use in diagnostic or therapeutic procedures.

Country of Origin :

USA

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