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Recombinant Human Lubricin

Lubricin Applications
(Also see Human Lubricin)

Description :

Lubricin/PRG4/MSF/SZP (GenBank NP_005798) is a mucinous glycoprotein which is both a lubricant of artcular cartilage and an anti-adhesive protein. It is expressed by superficial zone articular chondrocytes and synovial fibroblasts, and is also expressed by other organ systems including the liver, brain and heart. The protein has a molecular weight of 206 kDa measured by sedimentation analysis, yet due the post-translational modifications the apparent molecular weight on SDS-PAGE is 280-345 kDa. The variability may be due the 50% (w/w) post-translational modifications which are mainly O-linked β(1-3)Gal-GalNAc and α(2,3)NeuAc -β(1-3)Gal-GalNAc.

Catalog #:

001-Lubricin

Source :

CHO cells (Chinese Hamster Ovarian cells)

Formulation :

The sterile filtered solution was lyophilized from 1 x PBS, pH 7.0

Stability :

The lyophilized protein is stable for a few weeks at room temperature, but best stored at -800C. Reconstituted Human Lubricin should be stored in working aliquots at -200C.

Purity :

Greater than 95% by SDS-PAGE. Endotoxin level is less than 0.1 ng per μg (1EU/μg).

Reconstitution :

We recommend a quick spin followed by reconstitution in 1x PBS pH 7.2-7.4 to a concentration of 0.25 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 40C for 1 week or stored at -200C for future use.

Biological Activity :

Synoviocyte binding inhibition1 on Lubricin coated plates at 100 μg/ml, relative to uncoated plates, was 37% for recombinant Lubricin and 41% for Lubricin2 from synovial fluid.

Lubricin was tested for lubricating ability in a cartilage upon cartilage friction bearing3 using whole human Synoviocyte Fluids as a positive control. The μstatic was 0.076 ± 0.020 SD for Human Lubricin, and 0.093 ± 0.016 SD for recombinant Lubricin. The μstatic for phosphate buffered saline was 0.275 ± 0.037.

Usage:

For research purposes only.

Country of Origin :

USA



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1) Braut-Boucher F, Pichon J, Rat P, Adolphe M, Aubrey M, Font. A non-isotopic, highly sensitive, flourimetric, cell-cell adhesion microplate assay using calcein AM-labeled lymphocytes. J Immunol Methods 1995; 178: 41-51.

2) Jay GD, Tantravahi U, Britt DE, Barrach HJ, Cha C-J. Homology of lubricin and superficial zone protein (SZP): Products of megakaryocyte stimulating factor (MSF) gene expression by human synovial fibroblasts and articular chondrocytes localized to chromosome 1q25.  J Orthop Res 2001;19(4):677-687.

3) Schmidt TA, Gastelum NS, Nguyen QT, Schumacher BL, Sah RL. Boundary lubrication of articular cartilage: role of synovial fluid constituents. Arthritis Rheum 2007;56(3):882-91.


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