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Human Lubricin
Lubricin Applications
(Also see Recombinant Human Lubricin)
Description : |
Lubricin/PRG4/MSF/SZP (GenBank NP_005798) is a mucinous glycoprotein which is both a lubricant of artcular cartilage and an anti-adhesive protein. It is expressed by superficial zone articular chondrocytes and synovial fibroblasts, and is also expressed by other organ systems including the liver, brain and heart. The protein has a molecular weight of 206 kDa measured by sedimentation analysis, yet due the post-translational modifications the apparent molecular weight on SDS-PAGE is 280-345 kDa. The variability may be due the 50% (w/w) post-translational modifications which are mainly O-linked β(1-3)Gal-GalNAc and α(2,3)NeuAc -β(1-3)Gal-GalNAc. |
Catalog #: |
006-Lubricin |
Source : |
Human Synoviocytes |
Formulation : |
The sterile filtered solution was lyophilized from 1 x PBS, pH 7.0 |
Stability : |
The lyophilized protein is stable for a few weeks at room temperature, but best stored at -800C.
Reconstituted Human Lubricin should be stored in working aliquots at -200C. |
Purity : |
Greater than 95% by SDS-PAGE.
Endotoxin level is less than 0.1 ng per µg (1EU/µg). |
Reconstitution : |
We recommend a quick spin followed by reconstitution in 1x PBS pH 7.2-7.4 to a concentration of 0.25 mg/ml.
This solution can then be diluted into other aqueous buffers and stored at 40C
for 1 week or stored at -200C for future use. |
Biological Activity : |
Synoviocyte binding inhibition1 on Lubricin coated plates at 100 μg/ml, relative to uncoated plates, was 43% for synoviocyte Lubricin and 41% for Lubricin2 from synovial fluid. |
Usage: |
For research purposes only. |
Country of Origin : |
USA |
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1) Braut-Boucher F, Pichon J, Rat P, Adolphe M, Aubrey M, Font. A non-isotopic, highly sensitive, flourimetric, cell-cell adhesion microplate assay using calcein AM-labeled lymphocytes. J Immunol Methods 1995; 178: 41-51.
2) Jay GD, Tantravahi U, Britt DE, Barrach HJ, Cha C-J. Homology of lubricin and superficial zone protein (SZP): Products of megakaryocyte stimulating factor (MSF) gene expression by human synovial fibroblasts and articular chondrocytes localized to chromosome 1q25. J Orthop Res 2001;19(4):677-687.
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