Preparation: |
Monoclonal antibodies were produced from a
hybridoma resulting from the fusion of a mouse myeloma with B cells
obtained from a mouse immunized with purified, E. coli-derived,
recombinant human beta-NGF (human beta-Nerve Growth Factor).
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Purification: |
This IgG2b/Kappa antibody was purified by Protein
G affinity chromatography.
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Formulation: |
Lyophilized from a 0.2 mm filtered solution
in 0.5X phosphate-buffered saline (PBS) at pH 7.2.
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Catalog #: |
SBH-ABNGF
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Source: |
Mouse
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Stability: |
The lyophilized antibody is stable at room temperature for at least
one month and for greater than a year when
kept at -800C.
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Reconstitution: |
When reconstituted in sterile water to a concentration of
1 mg/ml the antibody is stable for at least six weeks
at 2-40C.
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Applications |
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ELISA: |
In a sandwich ELISA (assuming 100µl/well),
a concentration of 2 µg/ml of this antibody will detect at least
0.1 ng/well of recombinant human beta-NGF when used with conjunction
with PeproTech’s biotinylated antigen-affinity purified anti-human
ß-NGF (500-P85Bt) as the detection antibody at a concentration
of at least 0.5 µg/ml.
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Country of Origin: |
USA
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Usage: |
FOR RESEARCH USE ONLY. NOT FOR USE IN HUMANS.
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