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Recombinant Human Soluble IL-2 Receptor alpha (s-IL-2R-a)
Description : |
Human IL-2 exerts its biological effects via signaling through its receptor system,
IL-2R. IL-2 and its receptor (IL-2R) are required for T-cell proliferation and other
fundamental functions which are crucial of the immune response. IL-2R consists of 3
noncovalently linked type I transmembrane proteins which are the alpha (p55), beta (p75),
and gamma (p65) chains. The IL-2R alpha chain contains an extracellular domain of 219 amino acids,
a transmembrane domain of 19 amino acids, and an intracellular domain of 13 amino acids.
The secreted extracellular domain of IL-2R alpha (s-IL-2R-a), also called TAC-antigen,
is expressed on leukemia cells, lymphoma cells, approximately 10% NK cells, as well
as recently activated T and B cells. Recombinant human s-IL-2R-a is a 24.8 kDa protein
containing 219 amino acid residues consisting of only the extracellular domain of IL-2R alpha.
Due to glycosylation, IL-2R alpha has an approximate molecular weight of 31 kDa based on SDS-PAGE
gel and Mass Spectrometry. |
Catalog #: |
003-SIL2Ra |
Source : |
High-5 (BTI-Tn-5B1-4) Insect Cells |
Formulation : |
The sterile filtered solution was lyophilized from 1x PBS. |
Stability : |
The lyophilized protein is stable for a few weeks at room temperature, but
best stored at -200C. Reconstituted s-IL-2R alpha should be stored in working aliquots at 40C. |
Purity : |
Greater than 97% by SDS-PAGE and HPLC analyses.
Endotoxin level is less than 0.1 ng per µg (1EU/µg).
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Reconstitution : |
We recommend a quick spin followed by reconstitution in 1 x PBS,
pH 7.2 to a concentration of 0.1-1.0 mg/ml. This solution can then
be diluted into other aqueous buffers and stored at 40C for 1 week
or -200C for future use. |
Biological Activity : |
Determined by its ability to increase the proliferation
effect of IL-2 in murine CTLL-2 cells. In the presence of 1 ng/ml
of recombinant IL-2, the expected ED50 for this effect is between
0.5 - 1.5 ng/ml.
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PubMed Link : |
IL-2 receptor-alpha |
Usage: |
For research Purposes Only - Made using a cell BTI-Tn-5B1-4 under license from the Boyce Thompson Institute for Plant Research, Inc. Not for use in diagnostic or therapeutic procedures. |
Country of Origin : |
USA |
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