Analytical, ELISA, Simple Western Assays & Multiplex Services
SBH Sciences provides Contract Analytical, ELISA Development Kit services and Mutiplex services for the R&D and IVD community.
Among our services are HPLC, ELISA, RBA (receptor binding assay), ADA (anti-drug antibodies) and Western.
We have developed over 20 highly sensitive and accurate colorimetric sandwich ELISA Kits for the measurement of Cytokines and Biomarkers. Our ELISA Kits are already distributed worldwide.
Partial list of our kits is including the following cytokines (e.g., TNF-a, VEGF, IL-12 p70), Biomarkers / Cancer (e.g., Galectin-1, Galectin-3) and apoptosis markers (e.g., Bcl-2, Caspase-3).
We are providing custom service that will meet your specific needs. Moreover, we are providing in-house optimization to verify the impact of various serum / plasma / cell culture on your PK/PD study.
We will develop and provide a comprehensive instruction manual for performing the assay. It will include all the steps including: coating and wash solutions, blocking and secondary (conjugated) antibody. If requested, we can also perform all the testing of your samples at our facility.
Our expertise in the production of recombinant proteins and monoclonal antibodies (MAb) will enable us to produce your desired antigen and MAb and will significantly reduce the cost associated with the production / reagents. In addition, we will optimize the capture and detection antibodies to allow lower detection limit and high precision and accuracy measurement.
Automated Western Analysis Services
SBH Sciences and SBH Diagnostics are excited to introduce a new and innovative CRO protein analysis service in our CLIA registered lab for Pharmaceutical and Biotech Companies using traditional western blots in their research, development, and QC labs.
A major challenge in protein research is obtaining quantitative, reproducible, high content information quickly. SBH Diagnostics offers a new automated process (Simple WesternTM) using a novel capillary electrophoresis (CE) based immunodetection platform for protein separation and characterization. Proteins can be separated by size as in traditional PAGE Western or by their pI as in traditional CE or isoelectric focusing.
The SBH Diagnostics Service offers Simple WesternTM currently used in Pharma and Life sciences for vaccine development, cell lysate analysis (from tissue culture or tumors), identification and quantitation of tumor-associated proteins, focusing on key kinase inhibitor targets in precious clinical patient samples.
Benefits of the new SBH Diagnostic Western Service
- Provide quantitative reproducible protein analysis with CVs under 10%
- High throughput capability
- Able to multiplex and normalize to standard proteins
- Evaluate efficacy in cancer therapeutics against key cancer-associated target proteins in kinase signaling pathways
- Stratify patient populations for clinical trials
- Cost effective
The Simple Western™ Protein Analysis can provide:
- Protein quantification and characterization
- Protein Charge Heterogeneity
- Protein Formulation & Stability Testing
- Antibody and Antibody Drug-Conjugate Characterization
- Gel and Blot Documentation
- Immunoassay Multiplex Protein Detection
- Post-Translational Modification Characterization
The Caption below describes the Fully Automated process, for either molecular weight separation of proteins or separation by their pI.
Size/Charge-based characterization of proteins has been predominately performed by either SDS-PAGE/IEF/Western blot analysis or by capillary electrophoresis (CE). Each technique has advantages –Western blotting exploits high sensitivity as well as specificity of antibody binding, and CE offers high resolution and reproducibility. The sensitivity and resolution of the results obtained from either method is often challenged by the ability to preconcentrate or stack enough protein sample before separation. Simple Western is a new size-based or charge-based separation platform that runs Simple Western assays, combines for the first time the advantages of both Western blotting and CE into a single automated workflow. Optimization of stacking conditions results in a significant sensitivity and resolution increase using the Simple Western assay.
Learn more about our new Simple Western Applications and capabilities.
Please contact us to discuss your Western Applications and our service that can provide a fully automated characterization and quantification of proteins.
Custom Development and Testing Services of MultiAnalyte Profiling (MAP)
SBH Sciences is pleased to offer our new service : Multiplex Cytokines Custom Development and Testing Services based on our Cytokine expertise and the Luminex® xMAP Technology. The xMAP platform technology allows simultaneous quantification of MultiAnalyte Profiling including cytokines / chemokines / biomarkers in cell culture and biological samples.
We are offering tailor-made assay development of Multi Arrays usually based on already available commercial assays as well as additional of new innovative biological markers. We will develop the assay and will evaluate, verify and optimize the dynamic range, signal linearity, assay specificity and will ensure Multiplex compatibility. We are also providing Customer Sample Testing Services.
Our custom assays can be tailor-made for many applications including: Cancer Markers, Cardiac Markers, Growth Factors and Transcription Factors. It might assist to assess the mechanism of action for monoclonal antibodies, recombinant proteins, small molecule therapeutics across a range of disease states including cardiovascular, inflammation, immunology, oncology, and CNS disorders.
Our expertise in the production and purification will be applied as well.
For example, SBH Sciences can assist you in the isolation of specific
biomarker from natural sources or produce it as a recombinant protein
while simultaneously, we will also develop your specific MultiAnalyte
We are using the Luminex® 200™ analyzer.
(Luminex and Luminex 200 are registered trademarks of Luminex Corporation)
We also can perform Cell-Based assays for you samples using over 100 different cell lines. Please see our unique Cytokine Bioassay Services.
We believe in working together for our mutual success and encourage you to communicate with us.
Please contact us so we can discuss your specific needs, and ways in which SBH Sciences might be able to work with you. We will be happy to assist you.
Our expertise is your competitive edge!