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Cytokine Bioassay Services
Our cytokine bioassay capability is the most comprehensive one in the US and it is available for you. We are currently offering over two hundred bioassays in our two state-of-the-art cell culture facilities. SBH Sciences is pleased to offer to the scientific community a unique service, Cytokine Bioassays including: Proliferation and Neutralization, In Vitro Cytotoxicity, Complement Dependent Cytotoxicity (CDCC), Agonist and Antagonist Screening as well as Cytokine Release Assay. In addition, we are continually developing new assays and would be pleased to add others upon your request. We can assure strict confidentiality and superior execution which will be delivered on a timely basis and cost effective manner. The expected turnover time per assay is less than two weeks, and a written report including methods, results, and conclusions will be issued. SBH Sciences is determined to maintain the highest standards and deliver the highest quality products and services. SBH Sciences welcomes suggestions from researchers and encourage you to communicate with us about your particular research needs.
Most of the bioassays are based on a colorimetric method of measuring
cell activity using a non-radioactive substrate. This assay has been proven
to be equivalent to the [3H]thymidine incorporated assay (Cell
Signaling Applications Guide, Promega Corporation). The compound and the
standard (if required) are reconstituted, serial diluted, and incubated
with the cells over a 20 to 96 hour period.
BMPs (bone morphogenetic proteins) belong to the large group of growth
and differentiation factors from the TGF-beta superfamily. The bioassay
of the BMPs is based on measurement of alkaline phosphatase production
by ATDC-5 chondrogenic cells.
Innovative Bioassay
During the last 8 years we have built our expertise and currently we are providing the most comprehensive Cell-Based Cytokine assays in the US. Our array of cytokine bioassay services already accelerated discovery and development programs in numerous Biopharmaceuticals companies.
In addition, we have developed and implemented many NEW assays which become Industry STD. The list below is demonstrating our unique contribution to the Cytokine industry. We are proud to present our innovation and dedication and would be delighted continue assisting the R&D community. Please contact us with any question. Our mission is to be an extension of your research and development team!
- Activin-A and Activin B
- BAFF
- Flt-3 Ligand
- GDNF
- Myostatin and Myostatin Prepepdite
- Neuroserpin
Custom Bioassay Development
SBH Sciences provides complete custom bioassay development for the Pharmaceutical, Biotechnology and diagnostic industry. Our experience in bioassay development allows us to quickly develop custom assays to fit most applications.
Whenever you need to analyze the activity of novel compounds or neutralization capability of your antibody or receptor, please contact us. SBH Sciences can design, develop and validate the assay to meet your specific requirements.
Our in-vitro bioassays services will assist to assess the mechanism of action for monoclonal antibodies, recombinant proteins, small molecule therapeutics across a range of disease states including cardiovascular, inflammation, immunology, oncology, and CNS disorders.
Please see our current bioassays or contact us so we can discuss your needs, and ways in which SBH Sciences might be able to work with you.
Our mission is to be an extension of your research and development team.
Bioassay Under Development
We have a number of Bioassays in the final stages of development,
including: h-April and h-VEGF-B.
Cytotoxicity Assay
We currently have over 60 different cancer and non-cancer cell lines, as well as numerous Primary cells (e.g., HUVEC, HMVEC, NHDL). We already perform many anticancer drug screening and compare them to Industry STD (e.g., 5-Fluorouracil, Cisplatin, Doxorubicin, Irinotecan, Oxaliplatin, Paclitaxel). Please contact us with any request for cell-based Cytotoxicity test. We can perform any Non Radioactive Cytotoxicity Assay (e.g., The MultiTox-Glo Multiplex Cytotoxicity Assay, ApoGlow). We can order any new cell line and custom develop your assay as per your specific need.
Cost for Currently Available Assays
Proliferation or Cytotoxicity : $900 per one 96-well plate (2 samples
plus STD).
BMPs (alkaline phosphatase activity) : $950 per one 96-well plate (2 samples plus STD).
Neutralization assay : $1,100 per one 96-well plate (2 samples plus STD).
Agonist and Antagonist Screening : $1,100 per one 96-well plate (2 samples plus STD).
Cytokine Release Assay : $1,350 per one 96-well plate.
Volume discounts are available (for more than 25 assay plates per year).
Please contact us with your specific needs or any questions.
Cost for Development of New Assays
Development of a new bioassay: $4,000 (not including the cost of a new
cell line).
Generally, the development of new bioassays and the acquisition of new
cell lines will require approximately 6 to 8 weeks.
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