Recombinant Human B4GalT1 (β-1,4-Galactosyltransferase), soluble fragment




β1,4-galactosyltransferase 1 was the first mammalian glycosyltransferase to be isolated and cloned (1,2). β4GalT1 is a member of a homologous family of seven β1,4-galactosyltransferases, β4Gal-T1 – T7, that catalyze the transfer of galactose from UDP-Gal to acceptor sugars including GlcNAc (the acceptor substrate for β4GalT1), Glc and Xyl (3,4,5).  β4GalT1 exists as a membrane bound form and as a shed/secreted form deriving from proteolytic cleavage in the stalk region (6).  β4GalT1 may form a heterodimer with α-Lactalbumin, and the complex catalyzes the reaction of UDP-Gal + glucose -> lactose + UDP (7). 

  1. Shaper, K. et al. (1986) Proc. Nat. Acad. Sci. 83, 1573.
  2. Narimatsu, H. et al. (1986) Proc. Nat. Acad. Sci. 83, 4720.
  3. Almeida, R. et al. (1997) J. Biol. Chem. 272, 31979.
  4. Schwientek, T. et al. (1998) J. Biol. Chem. 273, 29331.
  5. Amado, M. et al. (1999) Biochim. Biophys. Acta. 1473, 35.
  6. Yamaguchi, N. and Fukuda, M.N. (1995) J. Biol. Chem. 270, 12170.
  7. Apert, H.E. et al. (1986) Biochem. Biophys. Res. Commun. 138, 224.

Catalog #:  SBH-003-B4GalT1

Source :  Insect Cells, Accession # P15291

Formulation:  Sterile filtered solution in 25mM Tris pH7.5 and 150mM NaCl, at a stock concentration of 440 ug/mL.

Stability:  Stable for 4 weeks at 4C.  Stable for 6 months at -80C.  Avoid repeated freeze-thaw cycles.

Purity:  Greater than 90% by SDS-PAGE.

Reconstitution:  N/A

Biological Activity:  Measured by transfer of galactose from UDP-galactose (UDP-Gal) to 4-Methylumbelliferyl N-acetyl-β-D-glucosaminide (GlcNAc-Mu, Sigma M2133), enzyme reactions containing:  25mM MES pH 7.4, 0.25% NP-40, 10mM MnCl2, 4mM UDP-Gal and 0.8mM GlcNAc-Mu.  Incubated at 37ºC for 30min. Specific activity > 2,500 pmol/min/mg.

UniProt  Link: B4GalT1 Human

Usage:  For research only. Not for use in diagnostic or therapeutic procedures.

Country of Origin:  USA

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