Recombinant Human B4GalT1 (β-1,4-Galactosyltransferase), soluble fragment
β1,4-galactosyltransferase 1 was the first mammalian glycosyltransferase to be isolated and cloned (1,2). β4GalT1 is a member of a homologous family of seven β1,4-galactosyltransferases, β4Gal-T1 – T7, that catalyze the transfer of galactose from UDP-Gal to acceptor sugars including GlcNAc (the acceptor substrate for β4GalT1), Glc and Xyl (3,4,5). β4GalT1 exists as a membrane bound form and as a shed/secreted form deriving from proteolytic cleavage in the stalk region (6). β4GalT1 may form a heterodimer with α-Lactalbumin, and the complex catalyzes the reaction of UDP-Gal + glucose -> lactose + UDP (7).
- Shaper, K. et al. (1986) Proc. Nat. Acad. Sci. 83, 1573.
- Narimatsu, H. et al. (1986) Proc. Nat. Acad. Sci. 83, 4720.
- Almeida, R. et al. (1997) J. Biol. Chem. 272, 31979.
- Schwientek, T. et al. (1998) J. Biol. Chem. 273, 29331.
- Amado, M. et al. (1999) Biochim. Biophys. Acta. 1473, 35.
- Yamaguchi, N. and Fukuda, M.N. (1995) J. Biol. Chem. 270, 12170.
- Apert, H.E. et al. (1986) Biochem. Biophys. Res. Commun. 138, 224.
Catalog #: SBH-003-B4GalT1
Source : Insect Cells, Accession # P15291
Formulation: Sterile filtered solution in 25mM Tris pH7.5 and 150mM NaCl, at a stock concentration of 440 ug/mL.
Stability: Stable for 4 weeks at 4C. Stable for 6 months at -80C. Avoid repeated freeze-thaw cycles.
Purity: Greater than 90% by SDS-PAGE.
Biological Activity: Measured by transfer of galactose from UDP-galactose (UDP-Gal) to 4-Methylumbelliferyl N-acetyl-β-D-glucosaminide (GlcNAc-Mu, Sigma M2133), enzyme reactions containing: 25mM MES pH 7.4, 0.25% NP-40, 10mM MnCl2, 4mM UDP-Gal and 0.8mM GlcNAc-Mu. Incubated at 37ºC for 30min. Specific activity > 2,500 pmol/min/mg.
UniProt Link: B4GalT1 Human
Usage: For research only. Not for use in diagnostic or therapeutic procedures.
Country of Origin: USA