Recombinant Human GalNAc-T5 (Polypeptide N-acetylgalactosaminyltransferase 5), soluble fragment

Description:

Polypeptide N-acetylgalactosaminyltransferase 5 (GalNAc-T5) catalyzes the transfer of N-acetylgalactosamine (GalNAc) from UDP-GalNAc to the hydroxyl group of serine and threonine residues, and was first cloned from rat (1).  Twenty GalNAc-T genes have been identified in humans and most have been shown to represent active GalNAc-Ts.  All isoforms are type II transmembrane proteins, with different but partly overlapping substrate preferences (2).  The GalNAc-Ts control the initiation of mucin-type O-linked glycosylation and determine the location and density of O-glycans in a protein (2).  Addition of GalNAc to an unglycosylated Ser/Thr residue creates the Tn antigen GalNAcα1-S/T, and subsequent addition of sialic acid by ST6GalNAc-I forms the cancer associated STn antigen (3).  GalNAc-T5 expression pattern is highly regulated, and is most abundant in salivary glands (1).  GalNAc-T5 has a preference for unglycosylated or monoglycosylated substrates (4).

1. Ten Hagen, K.G. et al. (1998) J. Biol. Chem. 273, 27749.
2.  Tian, E. & Ten Hagen, K.G. (2009) Glycocon. J. 26, 325.
3. Sewell, R. et al. (2006) J. Biol. Chem. 281, 3586.
4. Pratt, M.R. et al. (2004) Chem. & Biol. 11, 1009.

Catalog #:  SBH-003- GalNAc-T5

Source :  Insect Cells, Uniprot Accession # Q7Z7M9 

Formulation:  Sterile filtered solution in 25mM Tris pH7.5 and 150mM NaCl, at a stock concentration of  477ug/mL.

Stability:  Stable for 4 weeks at 4C.  Stable for 6 months at -80C.  Avoid repeated freeze-thaw cycles.

Purity:  Greater than 90% by SDS-PAGE.

Reconstitution:  N/A

Enzymatic Activity:  Measured by transfer of N-acetylgalatosamine (GalNac)  from UDP-Gal to the peptide EA2 (AnaSpec Inc, Catalog # 63841), as measured with a phosphatase-coupled assay (R&D Systems EA001).  Coupling assay reactions contained:  50mM Tris pH 7.5, 2mM MnCl₂, 0.5mM UDP-GalNAc, 0.25mM EA2, 4ug/mL ENTPD3/CD39L3, and 6ug/mL GalNAc-T5 (serially diluted 1:2 for 3pts).  Incubated at 37ºC for 30min. >500pmol/min/ug.

Pubmed  Link: GalNAc-T5 Human

Usage:  For research only. Not for use in diagnostic or therapeutic procedures.

Country of Origin:  USA