SMCxPRO™ Platform from MilliporeSigma

Single Molecule Counting (SMC™) for your ultrasensitive biomarker needs

SBH Sciences offers bioanalytical services for biomarker and large molecule analysis using the ultrasensitive Single Molecule Counting (SMC™) Technology. We offer a menu of MilliporeSigma SMC™ ultrasensitive biomarker assays and provide the following services:

• Biomarker Quantitation
• Pharmacokinetics and Pharmacodynamics (PK/PD)
• Immunogenicity (ADA)
• Assay Development

Verification Criteria for SMC™ Immunoassays
The SMC™ high sensitivity detection system addresses the needs of researchers who require highly quantitative, accurate, and robust biomarker assays. Our assays are rigorously developed and tested in matrix by multiple operators over multiple days.

A comprehensive set of criteria evaluating ultimate quantitative performance are used to qualify verified immunoassays, including:

Lower Limit of Quantification
Lowest point on the standard curve where CV is <20% and accuracy is within 20% of expected values.

Inter- and Intra- Assay Precision
Same samples run on multiple plates over multiple days to ensure reproducibility. Spiked and un-spiked samples report values within 20% across experiments.

Spike Recovery
Analyte added at the lower end of the standard curve to ensure accurate measurements for real samples. Minimum of 10 samples spiked with acceptable recovery between 80-120%.

Dilutional Linearity
Spiked samples diluted through at least 3 dilutions. Minimum of 10 samples spiked and diluted with acceptable recovery between 80-120%.

Dynamic Range
Assays target a quantifiable range of 3-4 logs.

Endogenous Range
Minimum of 10 samples from individual donors assessed for ability to quantify baseline biomarker levels.

Learn more about SMC™ technology at SigmaAldrich.com/smc

MilliporeSigma SMCTM Assay Offering the analysis of the following Analytes
Key: H=Human; M=Mouse; R=Rat; GP=Guinea Pig; Cy=Cynomolgus Monkey; C=Canine

Analyte	LLoQ  (pg/mL)1	Standard Curve Range (pg/mL)	Median Endogenous (pg/mL)2	Species3	Sample Types4	Sample volume (μL)
Phospho-α-Synuclein(Ser129)6,7	0.1	0.05-50	P: 2.74 S:  1.81 C:  0.64	H	P,S,C	20, 20, 50
Total-α-Synuclein6,7	1.95	0.49-500	P: 1885 S: 1240 C: 233	H	P,S,C	0.5, 0.5, 10
Phospho-Akt1 (Ser473)	0.98	1.0-1000	NA	H, M, R	L	varies8
Total Akt1	7.80	1.0-1000	NA	H, M, R	L	varies8
Amyloid beta 1-406,7	5.86	2.93-3000	Normal CSF:  1366 P:  152	H, M, R	C, P	5, 25
Amyloid beta 1-426,7	0.98	0.24-250	Normal CSF:  192 P:  15	H, M, R	C, P	5, 25
BDNF6,7	0.04	0.02-100	P:  783 S:  410 CSF:  0.13	H	C, P, S	100, 1, 1
cTnI 6,7	0.69	0.17-600	P:  3.04 S: 3.02	H, Cy, R, C, GP	P, S	100
G-CSF	0.08	0.02-20	17	H	P	10
GFAP6,7	0.12	0.06-300	P: 1.91 S: 1.93 C: 42.57	H	C, P, S	3.3, 100, 100
GLP-1 (active)	0.40	0.05-50	3.46	H, M, R, C	P	100
GLP-1 (total)	0.39	0.20-200	17.8	H, M, R, C	P	20
Glucagon6,7	0.78	0.20-200	44	H, M, R	P	10
GM-CSF	0.02	0.005-5	0.2	H	P	100
IFN-α26,7	0.031	0.015-80	P: 0.08 S: 0.08	H	P,S	100
IFN-β1	0.15	0.07-170	P: 0.35 S: 0.64	H	P,S	50
IFN-γ6,7	0.03	0.016-25	P:  1.4 S:  1.4	H	P, S	25
IL-1α26,7	0.12	0.06-60	P:  0.09 S:  0.10	H	P,S	100
IL-1β6,7	0.20	0.05-50	P:  0.11 S: 0.12	H	P,S	100
IL-2 (new version)	0.09	0.02-80	0.21	H	P, S	100
IL-21	0.20	0.11-250	0.53	H	S	50
IL-226,7	0.10	0.05-50	P:  1.51 S:  1.52	H	P,S	25
IL-236,7	0.10	0.1-100	P:  0.65 S:  0.46	H	P,S	100
IL-46,7	0.04	0.01-10	P:  0.18 S:  0.18	H	P,S	100
IL-56,7	0.04	0.05-50	P: 0.37 S:0.30	H	P,S	100
IL-66,7	0.08	0.01-50	P:  0.70 S: 0.69	H	P, S	75
IL-7	0.39	0.10-100	4.91	H	P, S	50
IL-8 / CXCL8	0.24	0.24-250	3.6	H	P, S	50
IL-106,7	0.10	0.10-100	P:  0.90 S:  0.89	H	P,S	100
IL-12p706,7	0.05	0.05-50	P:  0.29 S:  0.28	H	P, S	100
IL-136,7	0.04	0.02-100	P:  0.37 S:  0.33	H	P,S	100
IL-15	0.10	0.02-25	3.38	H	P, S	100
IL-17A/F Heterodimer6,7	1.17	0.60-600	P:  1.36 S:  3.20	H	P,S	50
IL-17A6,7	0.07	0.02-60	P:  0.31 S:  0.30	H	P, S	50
IL-17F6,7	0.20	0.10-100	P:  1.10 S:  1.18	H	P, S	50
IL-186,7	0.09	0.04-100	P: 138 S: 145	H	P,S	10
KIM-15	3.91	1.0-1000	P: 65 S: 75 U: 147	H	P, S, U	25, 25, 5
MCP-16,7	0.15	0.06-400	P: 682 S: 1021	H	P,S	1.56
NGFβ6,7	0.017	0.09-200	P: 1.20 S: 0.92	H	P,S	50
NPTX26,7	0.58	0.29-1000	P: 8268 S: 8161 C: 1677	H	P,S,C	0.1, 0.1, 0.3
Phospho-Tau(Thr181)6,7	0.94	0.23-240	P: 3.4 S: 2.0 C: 21	H	P,S,C	50, 100, 25
Total Tau6,7	2.34	1.17-1200	P: 27.1 S: 7.0 C: 88.91	H	P,S,C	50, 100, 25
TNFα	0.40	0.05-50	38.6	M	S	25
TNFα6,7	0.20	0.05-50	P:  0.85 S:  0.78	H	P,S	100
TSLP6,7	1.56	0.78-800	P: 5.04 S: 3.86	H	P,S	100
UCHL16,7	2.60	1.30-2000	P: 22.06 S: 20.49 C: 413	H	P,S,C	25,25,5
VEGF-A6,7	0.20	0.10-100	P:  48 S:  50	H	P,S	25
SARS-CoV-2 RBD IgG	NA9	NA9	NA9	H	P,S	2
SARS-CoV-2 S1 IgA	NA9	NA9	NA9	H	P,S	2

1 - LLoQ: Lowest point on standard curve that meets the following criteria for all sample types claimed: CV < 20% and accuracy within 20% of expected values, reproducible across at least three assay runs across three different operators.
2 - Median Endogenous: Minimum of 10 samples from individual donors assessed for ability to quantify baseline biomarker values.
3 - Optimized for first species type listed. Other listed species have been tested, but not optimized for peak performance.
4 - Optimized for use in sample type(s) listed. Key: P=Plasma; S=Serum; L=Lysate; U=Urine; C=Cerebrospinal Fluid (CSF)
5 - Plate-based assay
6 - Assay characteristic data is representative for both Erenna® and SMCxPRO™.
7 - Streamlined format kit.
8 - Sample volume varies based on dilution factor selected by user
9 - Qualitative assay

Please contact us so we can discuss your specific needs, we will be happy to assist you.