Recombinant Human ST6GalNAc1 (alpha-N-acetylgalactosaminide α-2,6-sialyltransferase 1), soluble fragment



Alpha-N-acetylgalactosaminide α-2,6-sialyltransferase 1 catalyzes the transfer of sialic acid N-acetylneuraminic acid (Neu5Ac) from cytidine 5'-monophosphono-N-acetylneuraminic acid (CMP-Neu5Ac) to O-linked GalNAc residues in an α-2,6 linkage (1).  ST6GalNAcI belongs to a large family of Golgi-membrane-bound sialyltransferases that is responsible for the biosynthesis of sialyated structures, and from which approximately 20 have been cloned to date (2).  The cancer associated sialyl-Tn (sTn) angtigen is formed by ST6GalNAcI, through the transfer of Neu5Ac to GalNAc resiudes on mucins (1,3,4).  Addition of a sialic acid to a glyco-protein effectively caps the glycan-chain, preventing further additions (5).  The presence of sialic acid residues extends the half-life of circulating proteins, and are considered to be important for a large variety of biotherapuetics (6).

  1.  Ikehara, Y. et al. (1999) Glycobio. 9, 1213.
  2. Harduin-Lepers, A. et al. (2001) Biochimie 83, 727.
  3. Marcos, N.T. et al. (2004) Cancer Res. 64, 7050.
  4. Sewell, R. et al. (2006) J. Biol. Chem. 281, 3586.
  5. Gill, D.J. et al. (2011) Trends Cell Biol. 21, 149.
  6. Varki, A. (2008) Trends Mol. Med. 14, 351.

Catalog #:  SBH-003-ST6GalNAc1

Source :  Insect Cells, Accession # Q9NSC7

Formulation:  Sterile filtered solution in 20mM Hepes pH7 and 100mM NaCl, at a stock concentration of 500 ug/mL.

Stability:  Stable for 4 weeks at 4C.  Stable for 6 months at -80C.  Avoid repeated freeze-thaw cycles.

Purity:  Greater than 90% by SDS-PAGE.

Reconstitution:  N/A

Biological Activity:  Measured by transfer of NeuAc from CMP-NeuAc to the Tn-peptide MEELGMAPALQPT(GalNAc)QGAMPAF, enzyme reactions containing: 25mM MES pH 6.5, 20mM EDTA, 1mM DTT, 2mM CMP-NeuAc and 10μg (400ug/mL final) Tn-peptide.  Final reaction contained 175ng of ST6GalNAcI. Incubated at 37ºC.  Specific activity > 50 pmol/min/μg.

PubMed  Link: ST6GalNAc1 Human

Usage:  For research only. Not for use in diagnostic or therapeutic procedures.

Country of Origin:  USA



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