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Cytokine, Cytokine Receptors, Anti-Cytokine and Chemokines Bioassay Services
Our cytokine and chemokines bioassay capability is the most comprehensive one worldwide. We currently offer 330 bioassays in our two state-of-the-art cell culture facilities. The types of assays available include:
- Cytokine-induced or reduced proliferation
- Antibody neutralization of cytokine-induced proliferation
- Complement Dependent Cytotoxicity (CDCC)
- Antibody Dependent Cytotoxicity (ADCC)
- Agonist and Antagonist Screening
- Cytokine Release (ELISA, LUMINEX)
- Chemokine functional assays based on GPCR activation of recombinant CHO cells.
Most of the bioassays are based on a colorimetric method of measuring cell activity using a non-radioactive substrate. This assay has been proven to be equivalent to the [3H] thymidine incorporated assay (Cell Signaling Applications Guide, Promega Corporation). The compound and the standard (if required) are reconstituted, serial diluted, and incubated with the cells over a 20 to 96 hour period. However, the Bone Morphogenetic Proteins (BMPs) bioassay is based on measurement of alkaline phosphatase production by ATDC-5 chondrogenic cells. BMPs belong to a large group of growth and differentiation factors from the TGF-beta superfamily.
At SBH Sciences, we are continually developing new assays for our customers and would be pleased to include additional ones at your request. We can assure strict confidentiality and superior execution which will be delivered in a time and cost effective manner. The expected turnaround time per assay is less than two weeks, and includes a written report containing methods, results, and conclusions. SBH Sciences is determined to maintain the highest standards and deliver the highest quality products and services. We welcome your suggestions and encourage you to communicate with us about your individual research requirements.
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