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MSD QuickPlex SQ 120 / S-Plex
The QuickPlex SQ 120 is an automated plate reader similar to the LUMINEX and ELLA designed for electrochemiluminescence immunoassays. It has a high sensitivity detection system detecting as low as 0.05pg/mL, a 5-log dynamic range and multiplexing capabilities. The main advantage the QuickPlex SQ 120 has over the ELLA and LUMINEX is its higher throughput. It can read a 96-well plate in 3 min versus Luminex and ELLA which can take 1.5 - 2 hours per plate.
There are 5 different assay types that the QuickPlex SQ 120 can run. The assay styles are R-Plex, S-Plex, T-Plex, U-Plex, and V-Plex. Each has a different optimization such as customization, validation, and ultrasensitive detection.
S-Plex
| Human |
| Analyte |
LLOD (fg/mL) |
LLOQ - ULOQ (fg/mL) |
| IL-4 |
0.54 |
4.9 – 3,300 |
| IL-6 |
1.1 |
1.3 – 4,400 |
| IL-10 |
1.4 |
9.8 – 12,000 |
| GM-CSF |
1.9 |
4.4 – 21,000 |
| IL-5 |
2.02 |
5.9 – 19,000 |
| IL-22 |
2.2 |
7.6 – 24,000 |
| IL-12p70 |
2.3 |
6.2 – 18,000 |
| TNF-β |
3.3 |
11 – 16,000 |
| IL-3 |
4.6 |
21 – 17,000 |
| IFN-α2a |
4.9 |
29 – 52,000 |
| IFN-γ |
5.3 |
16 – 15,000 |
| TNF-α |
6.8 |
12 – 42,000 |
| IL-2 |
7.3 |
21 – 30,000 |
| IL-9 |
8.6 |
39 – 68,000 |
| TSLP |
9.1 |
34 – 49,000 |
| IL-13 |
9.1 |
24 – 53,000 |
| IL-17A |
13 |
60 – 140,000 |
| IFN-β |
17 |
64 – 81,000 |
| IL-1β |
19 |
98 – 70,000 |
| G-CSF |
44 |
120 – 410,000 |
| Eotaxin-3 |
59 |
141 – 266,000 |
| SARS-CoV-2 N |
62 |
172 – 650,000 |
| SARS-CoV-2 Spike |
95 |
350 – 260,000 |
| NHP |
| Analyte |
LLOD (fg/mL) |
LLOQ - ULOQ (fg/mL) |
| IL-6 |
1.1 |
1.3 – 4,400 |
| IL-5 |
2.02 |
5.9 – 19,000 |
| TNF-β |
3.3 |
11 – 16,000 |
| IL-2 |
7.3 |
21 – 30,000 |
| IL-1β |
19 |
98 – 70,000 |
| G-CSF |
44 |
120 – 410,000 |
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