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MSD QuickPlex SQ 120 / T-Plex
The QuickPlex SQ 120 is an automated plate reader similar to the LUMINEX and ELLA designed for electrochemiluminescence immunoassays. It has a high sensitivity detection system detecting as low as 0.05pg/mL, a 5-log dynamic range and multiplexing capabilities. The main advantage the QuickPlex SQ 120 has over the ELLA and LUMINEX is its higher throughput. It can read a 96-well plate in 3 min versus Luminex and ELLA which can take 1.5 - 2 hours per plate.
There are 5 different assay types that the QuickPlex SQ 120 can run. The assay styles are R-Plex, S-Plex, T-Plex, U-Plex, and V-Plex. Each has a different optimization such as customization, validation, and ultrasensitive detection.
T-Plex
| Cytokine/Inflammation Panels |
| Species |
Name |
Analytes |
| Human |
TH1-TH2
10-Plex |
IFN-γ, IL-1β, IL-2, IL-4, IL-5, IL-8, IL-10,
IL-12p70, IL-13, TNF-α |
|
|
|
| Mouse |
TH1-TH2
9-Plex |
IFN-γ, IL-1β, IL-2, IL-4, IL-5, KC/GRO,
IL-10, IL12 total, TNF-α |
| Human |
ProInflammatory
9-Plex |
GM-CSF, IFN-γ, IL-1β, IL-2, IL-6, IL-8,
IL-10, IL-12p70, TNF-α |
|
|
|
| Mouse |
Proinflammatory
7-Plex |
IFN-γ, IL-1β, IL-6, IL-10, IL-12p70,
KC/GRO, TNF-α |
|
|
|
| Canine |
Proinflammatory
Panel 3 |
IL-2, IL-6, IL-8, TNF-α |
| Rat |
Inflammation
Panel 1 |
NGAL, TSP-1, TIMP-1, MCP-1 |
| Cyno |
Inflammation
Panel 3 |
MCP-1, NGAL, TIMP-1 |
| Human |
MMP 3-Plex Kit |
MMP-1, MMP-3, MMP-9 |
| Human |
Proinflammatory
7-Plex |
IFN-γ, IL-1β, IL-6, IL-8, IL-10, IL-12p70,
TNF-α |
| Toxicology/Injury Panels |
| Species |
Name |
Analytes |
| Rat |
Acute Phase Protein
Panel 1 |
A2M, AGP |
| Rat |
Inflammation Panel 1 |
NGAL, TSP-1, TIMP-1, MCP-1 |
| Cyno |
Inflammation Panel 3 |
MCP-1, NGAL, TIMP-1 |
| Rat |
Muscle Injury Panel 1 |
cTnI, cTnT, sTnI, FABP3, Myl3 |
| Rat |
Muscle Injury Panel 2 |
TIMP-1, CK |
| Mouse |
Muscle Injury Panel 3 |
cTnI, FABP3, Myl3, sTnI |
| Rat |
Liver Injury Panel 1 |
Arginase-1, αGST |
| Rat |
Kidney Injury Panel 1 |
Albumin, TIM-1, N-GAL,
Osteopontin |
| Human |
Kidney Injury
Panel 3 |
Calbindin, Clusterin, KIM-1,
Osteoactivin, TFF3, VEGF-A |
| Human |
Vascular Injury
Panel I |
sICAM-3, E-Selectin, P-Selectin,
Thrombomodulin |
| Human |
Growth Factor Panel I |
bFGF, VEGF-A, sFlt-1, PlGF |
| Human |
Growth Factor Panel II |
c-Kit, KDR |
| Human |
Bone Panel I |
ALP, Sclerostin, Osteoprotegrin |
| Human |
Bone Panel II |
Osteocalcin, Osteonectin,
Osteopontin |
| Activated/Total Panels |
| Species |
Analytes |
Human,
Mouse, Rat |
Akt pS473/Total |
| Human |
Caspase-3 (Cl. p20/p17)/Total |
| Human |
EGFR pY1173/Total |
| Human |
ErbB2 pY1248/Total |
Human,
Mouse, Rat |
ERK1/2 (pT202/pY204)/(pT185/pY187)/Total |
Human,
Mouse, Rat |
GSK-3β pS9/Total |
| Human |
JNK (pT183/pY185)/Total |
Human,
Mouse, Rat |
MEK1/2 (pS217/221)/Total |
Human,
Mouse, Rat |
p38 (pT180/pY182)/Total |
Human,
Mouse, Rat |
p53 Ub/Total |
Human,
Mouse, Rat |
p70S6K (pT421/pS424)/Total |
Human,
Mouse, Rat |
STAT5a,b pY694/Total |
| Secreted Analytes |
| Analyte |
Species |
| Adiponectin |
H, M, R |
| sAPPα |
H |
| sAPPβ (wild type) |
H, M |
sAPPβ (Swedish
variant) |
H |
|
|
| B2M |
R |
| BNP |
R |
| cAMP |
H, M, R |
| CHO (HCP) |
H |
| Clusterin |
R |
| C-Peptide |
M, R |
| Eotaxin-3 (CCL26) |
H |
| E-Selectin |
H |
| FABP3 |
H, M, R |
| FSH |
H |
| GIP |
H |
| GM-CSF |
H, M, R |
| HGF |
H |
| IFN-β |
H |
| IFN-γ |
M |
| IgA |
H, N |
| IgG |
H, N |
| IgM |
H, N |
| IL-1β |
M |
| IL-2 |
M |
| IL-4 |
M |
| IL-5 |
M |
| IL-6 |
M |
| IL-10 |
M |
| IL-12 |
M |
| IL-12/IL-23p40 |
M |
| IL-12p70 |
M |
| IL-17A |
H, M |
| IL-18 |
H |
| Insulin |
H, M, R |
| KC/GRO (CXCL1) |
M, R |
| KIM-1/TIM-1/HAVCR |
H, R |
| LBP |
H |
| Leptin |
H, M, R |
| MCP-1 (CCL2) |
M, R |
| M-CSF |
H |
| MIP-4 (CCL18) |
H |
| MMP-1 |
H |
| MMP-2 |
H |
| MMP-3 |
H |
| MMP-9 |
H |
| MMP-10 |
H |
Myeloperoxidase
(MPO) |
H |
| NT-proANP |
R |
| NT-proBNP |
H, R |
| Osteocalcin |
H |
| Osteonectin |
H |
| Osteopontin |
H, R |
| Osteoprotegerin |
H |
| Protein A |
H |
| E-Selectin |
H |
| P-Selectin |
H |
| PYY |
H, M, R |
| RANTES (CCL5) |
M |
| Resistin |
M, R |
| Tau |
B, H, M |
| Tau (pT231) |
H, M |
| TGF-β1 |
H |
| Thrombomodulin |
H |
| TIMP-1 |
H |
| TNF-α |
M |
| TNF-RI |
H |
| TNF-RII |
H |
| sTroponin I (sTnI) |
R |
| Troponin ITC Complex |
R |
| VEGF-R2 |
H |
| B=Bovine, |
M=Mouse, |
| R=Rat |
|
| Isotyping Panels |
| Species |
Name |
Analytes |
| Human, NHP |
Isotyping Panel 1 |
IgA, IgG, IgM |
| Mouse |
Isotyping Panel 1 |
IgA, IgG1, IgG2a, IgG2b, IgG3, IgM |
| Metabolic Panels |
| Species |
Analytes |
| Human, Rat |
Leptin, Insulin |
|
|
| Neurodegeneration Panels |
| Species |
Analytes |
| Human |
sAPPα
sAPPα, sAPPβ |
Human,
Mouse |
Tau (pT231)/Total Tau |
| Intracellular Signaling Panels |
| Species |
Name |
Analytes |
Human,
Mouse, Rat |
Akt Signaling Panel
(Phosphoprotein) |
pAkt, p70S6K, pGSK-3β |
Human,
Mouse, Rat |
Akt Signaling Panel (Total
Protein) |
Akt, p70S6K, GSK-3β |
Human,
Mouse, Rat |
Akt Signaling Panel II
(Phosphoprotein) |
pAkt, p70S6K, pGSK-3β,
pS6RP |
| Human |
Apoptosis Panel |
pp53, p53, Cl. Caspase-3,
Cl. PARP |
Human,
Mouse, Rat |
ERK-STAT3 Cascade
Panel |
pERK1/2, pMEK1/2, pSTAT3 |
| Human |
Insulin Signaling Panel
(Phosphoprotein) |
pIR, pIGF-1R, pIRS-1 |
| Human |
Insulin Signaling Panel
(Total Protein) |
IR, IGF-1R, IRS-1 |
Human,
Mouse, Rat |
MAP Kinase Panel
(Phosphoprotein) |
pERK1/2, pJNK, p38 |
| Human |
MAP Kinase Panel (Total
Protein) |
ERK1/2, JNK, p38 |
Human,
Mouse, Rat |
Phospho-STAT Panel |
pSTAT3, pSTAT4, pSTAT5a,b |
| Intracellular Analytes |
| Analyte |
Species |
| 4E-BP1 |
H |
| 4E-BP1 (pT37/46) |
H, M |
| Akt (pT308)* |
H, M |
| Caspase-3* |
H |
Caspase-3 (p20/p17)
(cleaved) |
H |
| CHOP |
H, M, R |
| c-Kit |
H, M, R |
| EGFR* |
H |
| EGFR (pY1173) |
H |
| eIF4E (pS209) |
H |
| ErbB2* |
H |
| ErbB2 (pY1248)* |
H |
| ERK-1/2 |
H, M, R |
ERK-1/2(pT202/
pY204)
(pT185/pY187) |
H, M, R |
| FOXO3a |
H, M |
| FOXO3a (pT32) |
H, M, R |
| FRS2 (pY196) |
H, M |
| FRS2 (pY436) |
H, M |
| GAPDH |
H, N |
| GSK-3α (pS21) |
H |
| GSK-3β (pS9)* |
H, M, R |
| HIF-1α |
H, M, R |
| Histone H3 (pS10) |
H, M, R |
| HSP70 |
H |
| IGF-1R (pY)** |
H |
| IRS-1 (S312) |
H |
| P-JNK* |
H |
MEK 1/2 (pS217/
pS221) |
H, M, R |
| NFκB (pS468) |
H, M, R |
| NFκB (pS536) |
H, M, R |
| p38 (pT180/pY182)* |
H, M, R |
| p53* |
H, M, R |
| p62 (SQSTM1) |
H, M, R |
| p70S6K |
H, M, R |
| p70S6K (pT389) |
H, M, R |
p70S6K (pT421/
pS424) |
H, M, R |
| PARP |
H |
| pDGFR-β (pY751) |
H, M |
| PERK |
H, M, R |
| PERK (pT980) |
R |
| PRAS40 |
H |
| PRAS40 (pT246) |
H, M, R |
| PSD-95 |
H, M, R |
| S6RP |
H, M, N, R |
| Sclerostin |
H |
| STAT3 |
H, M, R |
| STAT3 (pY705) |
H, M, R |
| STAT4 |
H, M, R |
| STAT4 (pY693) |
H, M, R |
| STAT5a/b |
H, M, R |
| STAT5a/b (pY694) |
H, M, R |
| VASP |
H |
| VASP (pS157) |
H |
| VEGFR2/KDR |
H |
| Wnt3a |
H, M, R |
| XBP-1 |
H, M, R |
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