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MSD QuickPlex SQ 120 / U-Plex
The QuickPlex SQ 120 is an automated plate reader similar to the LUMINEX and ELLA designed for electrochemiluminescence immunoassays. It has a high sensitivity detection system detecting as low as 0.05pg/mL, a 5-log dynamic range and multiplexing capabilities. The main advantage the QuickPlex SQ 120 has over the ELLA and LUMINEX is its higher throughput. It can read a 96-well plate in 3 min versus Luminex and ELLA which can take 1.5 - 2 hours per plate.
There are 5 different assay types that the QuickPlex SQ 120 can run. The assay styles are R-Plex, S-Plex, T-Plex, U-Plex, and V-Plex. Each has a different optimization such as customization, validation, and ultrasensitive detection.
U-Plex
| Human Biomarkers |
| Analyte | LLOD - ULOD, pg/mL |
| BAFF-R/TNFRSF13C |
1.4 – 14,000 |
| BCMA/TNFRSF17 |
0.13 – 600 |
| CD20 |
5.7 – 80,000 |
| CD27 |
0.41 – 3,400 |
| CD276/B7-H3 |
4.0 – 40,000 |
| CD28 |
14 – 144,000 |
| CD40L (soluble) |
0.23 – 1,800 |
| CTACK |
1.8 – 4,200 |
| CTLA-4 |
0.12 – 1,500 |
| ENA-78 |
0.53 – 3,900 |
| Eotaxin |
3.2 – 4,800 |
| Eotaxin-2 |
3.1 – 6,000 |
| Eotaxin-3 |
7.3 – 21,400 |
| EPO |
1.8 – 20,000 |
| FLT3L |
0.49 – 6,000 |
| Fractalkine |
100 – 181,000 |
| FGF (basic) |
2.1 – 1,200 |
| G-CSF |
1.6 – 20,400 |
| GITR/TNFRSF18 |
0.18 – 1,300 |
| GITRL/TNFSF18 |
0.09 – 1,000 |
| GM-CSF |
0.12 – 9,400 |
| gp130 (soluble) |
5.8 – 188,000 |
| Granzyme A |
0.49 – 3,700 |
| Granzyme B |
0.10 – 750 |
| GRO-α |
0.25 – 2,500 |
| HAVCR2/TIM-3 |
2.1 – 9,500 |
| I-309 |
6.8 – 3,000 |
| IFN-α2a |
4.0 – 42,400 |
| IFN-β |
3.1 – 100,000 |
| IFN-γ |
1.7 – 17,000 |
| IL-1α |
0.98 – 5,100 |
| IL-1β |
0.15 – 3,800 |
| IL-1RA |
1.7 – 5,000 |
| IL-2 |
0.70 – 1,900 |
| IL-2Rα |
10 – 55,000 |
| IL-3 |
11 – 16,000 |
| IL-4 |
0.08 – 2,100 |
| IL-5 |
0.24 – 4,000 |
| IL-6 |
0.33 – 2,000 |
| IL-7 |
1.5 – 7,000 |
| IL-8 |
0.15 – 2,200 |
| IL-9 |
0.14 – 1,500 |
| IL-10 |
0.14 – 3,700 |
| IL-12/IL-23p40 |
2.8 – 21,000 |
| IL-12p70 |
0.69 – 5,300 |
| IL-13 |
3.1 – 1,900 |
| IL-15 |
0.82 – 3,000 |
| IL-16 |
6.6 – 21,500 |
| IL-17A |
2.6 – 23,400 |
| IL-17A/F |
1.8 – 18,400 |
| IL-17B |
0.79 – 4,000 |
| IL-17C |
2.2 – 20,000 |
| IL-17D |
4.8 – 40,000 |
| IL-17E/IL-25 |
0.58 – 9,200 |
| IL-17F |
160 – 112,000 |
| IL-18 |
2.5 – 42,000 |
| IL-21 |
1.2 – 12,600 |
| IL-22 |
0.13 – 3,400 |
| IL-23 |
1.4 – 21,600 |
| IL-27 |
9.6 – 50,600 |
| IL-29/IFN-λ1 |
1.2 – 11,800 |
| IL-31 |
7.3 – 11,100 |
| IL-33 |
0.59 – 10,300 |
| IP-10 |
0.49 – 6,000 |
| I-TAC |
1.5 – 5,100 |
| LAG3 |
6.8 – 36,000 |
| MCP-1 |
0.74 – 6,600 |
| MCP-2 |
0.11 – 2,000 |
| MCP-3 |
0.79 – 5,000 |
| MCP-4 |
7.5 – 3,800 |
| M-CSF |
0.29 – 2,000 |
| MDC |
8.4 – 20,100 |
| MIF |
4.3 – 27,000 |
| MIP-1α |
7.7 – 4,200 |
| MIP-1β |
1.5 – 1,600 |
| MIP-3α |
1.8 – 20,800 |
| MIP-3β |
0.67 – 2,000 |
| MIP-5 |
0.34 – 30,000 |
| OX40/TNFRSF4 |
0.26 – 1,800 |
| PD1 (epitope 1) |
0.25 – 2,500 |
| PD1 (epitope 2) |
0.11 – 2,200 |
| PD-L1 (epitope 1) |
0.09 – 1,100 |
| PD-L2 |
3.9 – 41,000 |
| PlGF |
0.19 – 1,200 |
| RANKL/TNFSF11 |
1.8 – 4,000 |
| SDF-1α |
280 – 103,000 |
| TARC |
0.51 – 2,200 |
| TGF-β1 |
9.1 – 37,000 |
| TGF-β2 |
2.5 – 38,900 |
| TGF-β3 |
1.4 – 38,600 |
| Tie-2 |
2.9 – 29,000 |
| TIGIT |
0.36 – 3,500 |
| TLR1 |
4.4 – 37,000 |
| TNF-α |
0.54 – 3,700 |
| TNF-β |
0.47 – 4,300 |
| TPO |
19 – 40,400 |
| TRAIL |
0.66 – 10,000 |
| TSLP |
0.20 – 10,100 |
| VEGF-A |
2.0 – 4,900 |
| VEGF-D |
0.30 – 1,500 |
| YKL-40 |
0.39 – 5,000 |
|
|
| Mouse Biomarkers |
| Analyte | LLOD - ULOD, pg/mL |
| 6CKine/CCL21 |
1.5 – 4,000 |
| BAFF |
0.51 – 4,000 |
| BCA-1/BLC |
21 – 32,000 |
| CD40 |
2.6 – 8,000 |
| Eotaxin |
4.6 – 15,000 |
| EPO |
4.5 – 12,500 |
| GM-CSF |
0.16 – 1,000 |
| IFN-α |
140 – 100,000 |
| IFN-β |
5.2 – 6,000 |
| IFN-γ |
0.16 – 2,900 |
| IL-1β |
3.1 – 13,000 |
| IL-2 |
1.1 – 10,900 |
| IL-4 |
0.56 – 10,000 |
| IL-5 |
0.63 – 2,800 |
| IL-6 |
4.8 – 16,000 |
| IL-9 |
1.4 – 8,900 |
| IL-10 |
3.8 – 22,800 |
| IL-12/IL-23p40 |
1.4 – 20,400 |
| IL-12p70 |
48 – 89,000 |
| IL-13 |
2.7 – 22,800 |
| IL-15 |
24 – 131,000 |
| IL-16 |
3.6 – 6,300 |
| IL-17A |
0.30 – 2,100 |
| IL-17A/F |
0.61 – 10,600 |
| IL-17C |
2.3 – 45,600 |
| IL-17E/IL-25 |
1.6 –18,900 |
| IL-17F |
24 – 52,600 |
| IL-21 |
6.5 – 40,600 |
| IL-22 |
1.2 – 1,800 |
| IL-23 |
4.9 – 20,400 |
| IL-27p28/IL-30 |
8.7 – 73,300 |
| IL-31 |
45 – 66,300 |
| IL-33 |
2.2 – 36,000 |
| IP-10 |
0.51 – 4,900 |
| KC/GRO |
4.8 – 16,000 |
| MCP-1 |
1.4 – 1,400 |
| MCP-5/CCL12 |
0.14 – 1,500 |
| MDC |
13 – 10,000 |
| MIP-1α |
0.21 – 2,100 |
| MIP-1β |
13 – 30,800 |
| MIP-2 |
0.30 – 2,000 |
| MIP-3α |
0.10 – 2,500 |
| MMP-9 (total) |
49 – 80,000 |
| NGAL/LCN2 |
24 – 50,000 |
| RANTES |
0.72 – 2,000 |
| SDF-1α |
8.1 – 50,000 |
| TARC |
0.32 – 1,200 |
| TGF-β1 |
37 – 38,900 |
| TGF-β2 |
2.5 – 39,300 |
| TGF-β3 |
2.5 – 40,000 |
| TNF-α |
1.3 – 6,200 |
| TNF-RI |
0.46 – 2,000 |
| VEGF-A |
0.77 – 12,100 |
| NHP Biomarkers |
| Analyte | LLOD - ULOD, pg/mL |
| CTACK |
1.8 – 4,200 |
| ENA-78 |
0.36 – 3,900 |
| Eotaxin |
0.30 – 4,800 |
| Eotaxin-2 |
3.1 – 6,000 |
| Eotaxin-3 |
7.3 – 21,400 |
| FLT3L |
0.49 – 6,000 |
| Fractalkine |
100 – 181,000 |
| G-CSF |
1.5 – 20,400 |
| GM-CSF |
0.12 – 9,400 |
| GRO-α |
0.25 – 2,500 |
| I-309 |
6.8 – 3,000 |
| IFN-α2a |
1.7 – 40,800 |
| IFN-γ |
1.7 – 17,000 |
| IL-1α |
0.60 – 5,100 |
| IL-1β |
0.15 – 3,800 |
| IL-1RA |
1.7 – 5,000 |
| IL-2 |
0.70 – 1,900 |
| IL-4 |
0.06 – 2,100 |
| IL-5 |
0.24 – 4,000 |
| IL-6 |
0.33 – 2,000 |
| IL-7 |
1.5 – 7,000 |
| IL-8 |
0.15 – 2,200 |
| IL-9 |
0.14 – 1,500 |
| IL-10 |
0.14 – 3,700 |
| IL-12/IL-23p40 |
2.8 – 21,000 |
| IL-12p70 |
0.54 – 5,300 |
| IL-13 |
1.2 – 1,900 |
| IL-15 |
0.82 – 3,000 |
| IL-16 |
6.6 – 21,500 |
| IL-17A |
2.3 – 23,400 |
| IL-17A/F |
1.8 – 18,400 |
| IL-17B |
0.79 – 4,000 |
| IL-17C |
2.2 – 20,000 |
| IL-17D |
4.8 – 40,000 |
| IL-17F |
160 – 112,000 |
| IL-18 |
2.5 – 42,000 |
| IL-22 |
0.13 – 3,400 |
| IL-23 |
1.4 – 21,600 |
| IP-10 |
0.49 – 6,000 |
| I-TAC |
1.5 – 2,000 |
| MCP-1 |
0.74 – 6,600 |
| MCP-2 |
0.11 – 2,000 |
| MCP-3 |
0.79 – 5,000 |
| MCP-4 |
7.5 – 3,800 |
| M-CSF |
0.29 – 2,000 |
| MDC |
8.4 – 20,100 |
| MIF |
4.3 – 27,000 |
| MIP-1α |
7.7 – 4,200 |
| MIP-1β |
1.5 – 1,600 |
| MIP-3α |
0.27 – 20,800 |
| MIP-3β |
0.67 – 2,000 |
| MIP-5 |
0.34 – 30,000 |
| SDF-1α |
18 – 103,000 |
| TARC |
0.51 – 2,200 |
| TGF-β1 |
9.1 – 37,000 |
| TGF-β2 |
2.5 – 38,900 |
| TGF-β3 |
1.4 – 38,600 |
| TNF-α |
0.54 – 3,700 |
| TNF-β |
0.47 – 4,300 |
| TPO |
19 – 40,400 |
| TRAIL |
0.66 – 10,000 |
| VEGF-A |
2.0 – 4,900 |
| YKL-40 |
0.39 – 5,000 |
| U-PLEX Metabolic Human Combinations |
| Name (Cat. No.) |
Analytes |
| Metabolic Group 1 87-Plex (K15280K) |
BAFF, BDNF, β-NGF, C-Peptide, CTACK (CCL27), ENA-78 (CXCL5), Eotaxin (CCL11), Eotaxin-2 (CCL24), Eotaxin-3 (CCL26),
EPO, FGF-21, FGF-23, FLT3L, FSH, Fractalkine (CX3CL1), G-CSF, Ghrelin (active), Ghrelin (total), GIP (active), GIP (inactive),
GIP (total), GLP-1 (active), GLP-1 (inactive), GLP-1 (total), Glucagon, GM-CSF, GRO-α (CXCL1), I-309 (CCL1), IFN-α2a,
IFN-β, IFNγ, IL1α, IL-1β, IL-1RA, IL-2, IL-2Rα, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8 (CXCL8), IL9, IL-10, IL12/IL-23p40, IL-12p70,
IL-13, IL-15, IL-16, IL-17A, IL-17A/F, IL17C, IL-17D, IL-17E/IL-25, IL17F, IL-18, IL-21, IL-22, IL-23, IL-27, IL29/IFN-λ1,
IL-31, IL-33, Insulin, IP-10 (CXCL10), Leptin, LH, MCP-1 (CCL2), MCP-2 (CCL8), MCP-4 (CCL13), M-CSF, MDC (CCL22),
MIF, MIP-1α (CCL3), MIP-5, PP, Proinsulin, PYY (total), SDF-1α (CXCL12), TARC (CCL17), TNF-α, TNF-β, TPO, TRAIL,
TSLP, VEGFA, YKL-40 |
| Adipokine Combo 1 (K15276K) |
BDNF, β-NGF, IL-1β, IL-6, IL-8, IL-10, Insulin, Leptin, MCP-1, TNF-α |
| Diabetes Combo 1 (K15274K) |
C-Peptide, GIP (total), GLP-1 (total), Glucagon, Insulin, Leptin, PYY (total) |
| Diabetes Combo 2 (K15275K) |
C-Peptide, GIP (total), GLP-1 (active), Glucagon, Insulin, Leptin, PYY (total) |
| Metabolic Combo 1 (K15281K) |
BAFF, BDNF, β-NGF, C-Peptide, FGF-21, FGF-23, FSH, Ghrelin (active), Ghrelin (total), GIP (active), GIP (inactive),
GIP (total), GLP-1 (active), GLP-1 (inactive), GLP-1 (total), Glucagon, Insulin, Leptin, LH, PP, Proinsulin, PYY (total) |
| Metabolic 2-Plex Combo 1 (K15282K) |
Insulin, Leptin |
| Metabolic 3-Plex Combo 1 (K15283K) |
GLP-1 (active), Glucagon, Insulin |
| Metabolic 4-Plex Combo 1 (K15284K) |
GLP-1 (active), Glucagon, Insulin, Leptin |
| Microbiome Combo 1 (K15441K) NEW |
Ghrelin (total), GLP-1 (total), Glucagon, Insulin, Leptin, PP, PYY (total) |
| Obesity Combo 1 (K15277K) |
BDNF, FGF-21, Ghrelin (total), Glucagon, Leptin |
| Obesity Combo 2 (K15278K) |
C-Peptide, FGF-23, Ghrelin (total), GLP-1 (total), Insulin, Leptin, PYY (total) |
|
|
| U-PLEX Metabolic Mouse Combinations |
| Name (Cat. No.) |
Analytes |
|
|
| Metabolic Group 1 58-Plex (K15317K) |
BBAFF, BCA-1/BLC, BDNF, CD40, C-Peptide, Eotaxin, EPO, FGF-21, Ghrelin (active), Ghrelin (total), GLP-1 (active), GLP-1 (inactive), GLP-1 (total), Glucagon, GM-CSF, IFN-α, IFN-β, IFN-γ, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12/IL-23p40, IL-12p70, IL-13, IL-15, IL-16, IL-17A, IL-17A/F, IL-17C, IL-17E/IL-25, IL-17F, IL-21, IL-22, IL-23, IL-27p28/IL-30, IL-31, IL-33, Insulin, IP-10, KC/GRO, Leptin, MCP-1, MCP-5/Ccl12, MDC, MIP-1α, MIP-1β, MIP-2, MIP-3α, MMP-9 (total), PYY (total), RANTES, TARC, TNF-α, VEGF-A |
|
|
| Adipokine Combo 1 (K15299K) |
BDNF, IL-1β, IL-6, IL-10, Insulin, Leptin, MCP-1, TNF-α |
| Diabetes Combo 1 (K15298K) |
C-Peptide, GLP-1 (total), Glucagon, Insulin, Leptin, PYY (total) |
| Gut Hormone Combo 1 (K15307K) |
Ghrelin (active), GLP-1 (active), Glucagon, Insulin, Leptin, PYY (total |
| Metabolic Combo 1 (K15297K) |
BAFF, BDNF, C-Peptide, FGF-21, Ghrelin (active), Ghrelin (total), GLP-1 (active), GLP-1 (inactive), GLP-1 (total),
Glucagon, Insulin, Leptin, PYY (total) |
|
Glucagon, Insulin, Leptin, PYY (total) |
| Metabolic 2-Plex Combo 1 (K15302K) |
Insulin, Leptin |
| Metabolic 2-Plex Combo 2 (K15303K) |
Glucagon, Insulin |
| Metabolic 3-Plex Combo 1 (K15304K) |
GLP-1 (total), Glucagon, Insulin |
| Metabolic 3-Plex Combo 2 (K15305K) |
GLP-1 (active), Glucagon, Insulin |
| Metabolic Hormones Combo 1 (K15306K) |
C-Peptide, Ghrelin (active), GLP-1 (active), Glucagon, IL-6, Insulin, Leptin, MCP-1, PYY (total), TNF-α |
| Obesity Combo 1 (K15300K) |
BDNF, FGF-21, Ghrelin (total), Glucagon, Leptin |
| Obesity Combo 2 (K15301K) |
C-Peptide, Ghrelin (total), GLP-1 (total), Insulin, Leptin, PYY (total) |
|
|
| U-PLEX Metabolic Rat Combinations |
| Name (Cat. No.) |
Analytes |
| Metabolic Combo 1 (K15308K) |
BDNF, C-Peptide, FGF-21, Ghrelin (active), Ghrelin (total), GLP-1 (active), GLP1 (inactive), GLP-1 (total), Glucagon, Insulin, Leptin, PYY (total) |
| Diabetes Combo 1 (K15309K) |
C-Peptide, GLP-1 (total), Glucagon, Insulin, Leptin, PYY (total) |
| Metabolic 2-Plex Combo 1 (K15312K) |
Insulin, Leptin |
| Metabolic 2-Plex Combo 2 (K15313K) |
Glucagon, Insulin |
| Metabolic 3-Plex Combo 1 (K15314K) |
GLP-1 (total), Glucagon, Insulin |
| Metabolic 3-Plex Combo 2 (K15315K) |
GLP-1 (active), Glucagon, Insulin |
| Metabolic Hormones Combo 1 (K15316K) |
C-Peptide, Ghrelin (active), GLP-1 (active), Glucagon, Insulin, Leptin, PYY |
| Obesity Combo 1 (K15310K) |
BDNF, FGF-21, Ghrelin (total), Glucagon, Leptin |
| Obesity Combo 2 (K15311K) |
C-Peptide, Ghrelin (total), GLP-1 (total), Insulin, Leptin, PYY (total) |
| U-PLEX Metabolic Group I Human Biomarkers |
| Analyte |
LLOD - ULOD |
Units |
| BAFF |
0.05 – 500 |
pg/mL |
| BDNF* |
0.72 – 2,000 |
pg/mL |
| β-NGF* |
0.05 – 498 |
pg/mL |
| C-Peptide* |
14 – 7,610 |
pg/mL |
| FGF-21 |
2.8 – 8,230 |
pg/mL |
| FGF-23 |
0.75 – 3,000 |
pg/mL |
| FSH |
9.0 – 75,000 |
µIU/mL |
| Ghrelin (active) |
13 – 7,160 |
pg/mL |
| Ghrelin (total) |
1.7 – 2,710 |
pg/mL |
| GIP (active) |
1.3 – 1,920 |
pg/mL |
| GIP (inactive) |
27 – 12,500 |
pg/mL |
| GIP (total) |
3.7 – 12,500 |
pg/mL |
| GLP-1 (active) |
0.01 – 57 |
pM |
| GLP-1 (inactive) |
1.5 – 576 |
pM |
| GLP-1 (total) |
0.59 – 576 |
pM |
| Glucagon* |
0.13 – 156 |
pM |
| Insulin* |
0.32 – 736 |
µIU/mL |
| Leptin* |
14 – 47,500 |
pg/mL |
| LH* |
1.6 – 27,700 |
µIU/mL |
| PP |
0.19 – 1,830 |
pg/mL |
| Proinsulin* |
0.05 – 130 |
pM |
| PYY (total) |
2.7 – 2,260 |
pg/mL |
|
|
|
|
|
|
| U-PLEX Metabolic Group I Mouse Biomarkers |
| Analyte |
LLOD - ULOD |
Units |
| BDNF* |
0.72 – 2,000 |
pg/mL |
| C-Peptide |
220 – 125,000 |
pg/mL |
| FGF-21 |
2.8 – 8,230 |
pg/mL |
| Ghrelin (active) |
13 – 7,160 |
pg/mL |
| Ghrelin (total) |
1.7 – 2,710 |
pg/mL |
| GLP-1 (active) |
0.14 – 57.0 |
pM |
| GLP-1 (inactive) |
1.5 – 576 |
pM |
| GLP-1 (total) |
0.59 – 576 |
pM |
| Glucagon* |
0.13 – 156 |
pM |
| Insulin |
3.0 – 5,500 |
µIU/mL |
| Leptin |
11 – 50,000 |
pg/mL |
| PYY (total) |
1.1 – 4,000 |
pg/mL |
| U-PLEX Metabolic Group I Rat Biomarkers |
| Analyte |
LLOD - ULOD |
Units |
| BDNF* |
0.72 – 2,000 |
pg/mL |
| C-Peptide |
220 – 125,000 |
pg/mL |
| FGF-21 |
2.8 – 8,230 |
pg/mL |
| Ghrelin (active) |
13 – 7,160 |
pg/mL |
| Ghrelin (total) |
1.7 – 2,710 |
pg/mL |
| GLP-1 (active) |
0.14 – 57.0 |
pM |
| GLP-1 (inactive) |
1.5 – 576 |
pM |
| GLP-1 (total) |
0.59 – 576 |
pM |
| Glucagon* |
0.13 – 156 |
pM |
| Insulin |
3.0 – 5,500 |
µIU/mL |
| Leptin |
11 – 50,000 |
pg/mL |
| PYY (total) |
1.1 – 4,000 |
pg/mL |
|
|
