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MSD QuickPlex SQ 120 / V-Plex
The QuickPlex SQ 120 is an automated plate reader similar to the LUMINEX and ELLA designed for electrochemiluminescence immunoassays. It has a high sensitivity detection system detecting as low as 0.05pg/mL, a 5-log dynamic range and multiplexing capabilities. The main advantage the QuickPlex SQ 120 has over the ELLA and LUMINEX is its higher throughput. It can read a 96-well plate in 3 min versus Luminex and ELLA which can take 1.5 - 2 hours per plate.
There are 5 different assay types that the QuickPlex SQ 120 can run. The assay styles are R-Plex, S-Plex, T-Plex, U-Plex, and V-Plex. Each has a different optimization such as customization, validation, and ultrasensitive detection.
V-Plex
| Human |
| Analyte |
LLOD
(pg/mL) |
LLOQ-ULOQ
(pg/mL) |
| Aβ38 (6E10) |
16.7 |
60 – 8,480 |
| Aβ40 (6E10) |
9.97 |
50 – 7,000 |
| Aβ42 (6E10) |
0.368 |
3.13 – 1,270 |
| Aβ38 (4G8) |
22.2 |
60 – 7,500 |
| Aβ40 (4G8) |
5.41 |
20 – 6,000 |
| Aβ42 (4G8) |
0.516 |
2.5 – 1,270 |
| Aβ42 |
0.33 |
3.0 – 8,000 |
| C-Peptide |
4.72 |
19 – 3,150 |
| CRP |
1.33 |
27.6 – 49,600 |
| Eotaxin |
3.26 |
12.3 – 1,120 |
| Eotaxin-3 |
1.77 |
10.2 – 3,750 |
| FGF (basic) |
0.09 |
2.6 – 1,780 |
| Flt-1/VEGFR-1 |
0.90 |
10 – 6,410 |
| GIP (active) |
0.233 |
3.18 – 390 |
| GM-CSF |
0.16 |
0.842 – 750 |
| ICAM-1 |
1.94 |
15.0 – 32,700 |
| IFN-γ |
0.37 |
1.76 – 938 |
| IL-1α |
0.09 |
2.85 – 278 |
| IL-1β |
0.05 |
0.646 – 375 |
| IL-1RA |
1.12 |
9.19 – 650 |
| IL-2 |
0.09 |
0.890 – 938 |
| IL-3 |
2.37 |
12.6 – 1,950 |
| IL-4 |
0.02 |
0.218 – 158 |
| IL-5 |
0.14 |
4.41 – 562 |
| IL-6 |
0.06 |
0.633 – 488 |
| IL-7 |
0.12 |
0.546 – 563 |
| IL-8 |
0.07 |
0.591 – 375 |
| IL-8 (HA*) |
95.6 |
713 – 43,400 |
| IL-9 |
0.311 |
2.23 – 975 |
| IL-10 |
0.04 |
0.298 – 233 |
| IL-12/IL-23p40 |
0.33 |
1.32 – 2,250 |
| IL-12p70 |
0.11 |
1.22 – 315 |
| IL-13 |
0.24 |
4.21 – 353 |
| IL-15 |
0.15 |
0.774 – 525 |
| IL-16 |
2.83 |
19.1 – 1,870 |
| IL-17A |
0.31 |
3.19 – 3,650 |
| IL-17A (Gen. B) |
0.413 |
5.86 – 1,950 |
| IL-17A/F |
0.930 |
7.57 – 3,900 |
| IL-17B |
0.185 |
1.12 – 1,040 |
| IL-17C |
0.682 |
3.84 – 1,620 |
| IL-17D |
3.87 |
11.2 – 5,200 |
| IL-21 |
0.193 |
1.65 – 650 |
| IL-22 |
0.27 |
2.78 – 325 |
| IL-23 |
0.274 |
4.60 – 3,250 |
| IL-27 |
4.2 |
38.7 – 13,000 |
| IL-31 |
0.446 |
4.22 – 650 |
| Insulin |
0.006 |
0.07 – 19.5
(µIU/ml) |
| IP-10 |
0.37 |
1.37 – 500 |
| Leptin |
5.78 |
37.7 – 7,150 |
| MCP-1 |
0.090 |
1.09 – 375 |
| MCP-4 |
1.69 |
10.0 – 469 |
| MDC |
1.22 |
88.3 – 7,500 |
| MIP-1α |
3.02 |
13.8 – 743 |
| MIP-1β |
0.17 |
1.88 – 750 |
| MIP-3α |
0.05 |
0.588 - 325 |
| PlGF |
0.21 |
1.5 – 800 |
| PP |
0.043 |
0.41 – 325 |
| SAA |
10.9 |
54.1 – 138,000 |
| TARC |
0.22 |
3.32 – 1,120 |
| Tau |
10.18 |
30 – 8,000 |
| Tie-2 |
31.3 |
396 – 63,400 |
| TNF-α |
0.04 |
0.690 – 248 |
| TNF-β |
0.08 |
0.465 – 458 |
| TSLP |
0.063 |
0.460 – 325 |
| VCAM-1 |
6.00 |
37.6 – 32,000 |
| VEGF-A |
1.12 |
7.70 – 562 |
| VEGF-C |
9.91 |
146 – 17,500 |
| VEGF-D |
4.36 |
67.1 – 18,800 |
| Non-Human Primate (NHP) |
| Analyte |
LLOD
(pg/mL) |
LLOQ - ULOQ
(pg/mL) |
| C-Peptide |
4.72 |
19 – 3,150 |
| Eotaxin-3 |
1.77 |
10.2 – 3,750 |
| GIP (active) |
0.233 |
3.18 – 390 |
| GM-CSF |
0.16 |
0.842 – 750 |
| IFN-γ |
0.37 |
1.76 – 938 |
| IL-1β |
0.05 |
0.646 – 375 |
| IL-2 |
0.09 |
0.890 – 938 |
| IL-5 |
0.14 |
4.41 – 562 |
| IL-6 |
0.06 |
0.633 – 488 |
| IL-7 |
0.12 |
0.546 – 563 |
| IL-8 |
0.07 |
0.591 – 375 |
| IL-8 (HA) |
95.6 |
713 – 43,400 |
| IL-10 |
0.04 |
0.298 – 233 |
| IL-12/IL-23p40 |
0.33 |
1.32 – 2,250 |
| IL-15 |
0.15 |
0.774 – 525 |
| IL-16 |
2.83 |
19.1 – 1,870 |
| IL-17A |
0.31 |
3.19 – 3,650 |
| Insulin |
0.006 |
0.07 – 19.5
(µIU/ml) |
| IP-10 |
0.37 |
1.37 – 500 |
| MCP-1 |
0.09 |
1.09 – 375 |
| MCP-4 |
1.69 |
5.13 – 469 |
| MDC |
1.22 |
88.3 – 7,500 |
| MIP-1α |
3.02 |
13.8 – 743 |
| MIP-1β |
0.17 |
1.02 – 750 |
| PP |
0.043 |
0.41 – 325 |
| TARC |
0.22 |
3.32 – 1,120 |
| TNF-β |
0.08 |
0.465 – 458 |
| VEGF-A |
1.12 |
7.70 – 562 |
|
|
|
| Canine |
| Analyte |
LLOD
(pg/mL) |
LLOQ - ULOQ
(pg/mL) |
| Insulin |
0.006 |
0.07 – 19.5
(µIU/ml) |
| GIP (active) |
0.233 |
3.18 – 390 |
| TNF-α |
0.72 |
9.1 – 793 |
|
|
|
| Rat |
| Analyte |
LLOD
(pg/mL) |
LLOQ - ULOQ
(pg/mL) |
| Aβ38 (4G8) |
22.2 |
3.18 - 390 |
| Aβ40 (4G8) |
5.41 |
20 – 6,000 |
| Aβ42 (4G8) |
0.516 |
2.5 – 1,270 |
| IFN-γ |
0.65 |
39.7 – 3,750 |
| GIP (active) |
0.233 |
3.18 – 390 |
| IL-1β |
6.92 |
102 – 8,100 |
| IL-4 |
0.69 |
8.0 – 723 |
| IL-5 |
14.1 |
82 – 3,000 |
| IL-6 |
13.8 |
96.9 – 8,550 |
| IL-10 |
16.4 |
163 – 15,670 |
| IL-13 |
1.97 |
12.5 – 1,080 |
| KC/GRO |
1.04 |
21.7 – 728 |
| TNF-α |
0.72 |
9.1 – 793 |
|
|
|
|
|
|
| Mouse |
| Analyte |
LLOD
(pg/mL) |
LLOQ - ULOQ
(pg/mL) |
| Aβ38 (4G8) |
22.2 |
60 – 7,500 |
| Aβ40 (4G8) |
5.41 |
20 – 6,000 |
| Aβ42 (4G8) |
0.516 |
2.5 – 1,270 |
| IFN-γ |
0.04 |
0.39 – 570 |
| IL-1β |
0.11 |
0.72 – 1,030 |
| IL-2 |
0.22 |
1.03 – 1,570 |
| IL-4 |
0.11 |
0.818 – 1,060 |
| IL-5 |
0.06 |
0.302 – 590 |
| IL-6 |
0.61 |
7.61 – 3,140 |
| IL-9 |
3.84 |
21.9 – 2,600 |
| IL-10 |
0.94 |
7.26 – 2,030 |
| IL-12p70 |
9.95 |
179 – 20,600 |
| IL-15 |
16 |
43.2 – 26,000 |
| IL-17A/F |
0.23 |
1.39 – 1,620 |
| IL-27p28/IL-30 |
1.39 |
5.91 – 6,500 |
| IL-33 |
0.36 |
1.85 – 1,950 |
| IP-10 |
0.32 |
2.15 – 650 |
| KC/GRO |
0.24 |
3.29 – 1,230 |
| MCP-1 |
0.672 |
4.42 – 325 |
| MIP-1α |
0.081 |
0.380 – 390 |
| MIP-2 |
0.053 |
0.580 – 423 |
| TNF-α |
0.13 |
0.97 – 403 |
|
|
|
| Human/NHP/Mouse/Rat/Canine |
|
|
|
| Analyte |
LLOD
(pM) |
LLOQ - ULOQ
(pM) |
| Glucagon |
0.015 |
0.33 – 52 |
| GLP-1 Active*** |
0.02 |
0.30 – 120 |
| GLP-1 Total*** |
0.017 |
0.18 – 120 |
| Preconfigured V-PLEX Kits |
| Species |
Name (Cat. No.) |
Analytes |
| Human |
Biomarker 54-Plex Kit (K15248G) |
CRP, Eotaxin, Eotaxin-3, FGF (basic), Flt-1, GM-CSF, ICAM-1, IFN-γ, IL-1α, IL-1β, IL-1RA, IL-2, IL-3,
IL-4, IL-5, IL-6, IL-7, IL-8, IL-8 (high abundance), IL-9, IL-10, IL-12p 70, IL-12/IL-23p40, IL-13, IL-15,
IL-16, IL-17A, IL-17A/F, IL-17C, IL-17D, IL-21, IL-22, IL-23, IL-27, IL-31, IP-10, MCP-1, IL-17B,
MCP-4, MDC, MIP-1α, MIP-1β, MIP-3α, PIGF, SAA, TARC, Tie-2, TNF-α, TNF-β, TSLP, VCAM-1, VEGF-A,
VEGF-C, VEGF-D |
| Biomarker 46-Plex Kit (K15088G) |
CRP, Eotaxin, Eotaxin-3, FGF (basic), Flt-1, GM-CSF, ICAM-1, IFN-γ, IL-1α, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-7,
IL-8, IL-8 (HA*), IL-10, IL-12p70, IL-12/IL-23p40, IL-13, IL-15, IL-16, IL-17A, IL-21, IL-22, IL-23, IL-27, IL-31,
IP-10, MCP-1, MCP-4, MDC, MIP-1α, MIP-1β, MIP-3α, PIGF, SAA, TARC, Tie-2, TNF-α, TNF-β, VCAM-1,
VEGF-A, VEGF-C, VEGF-D |
| Biomarker 40-Plex Kit (K15209G) |
CRP, Eotaxin, Eotaxin-3, FGF (basic), Flt-1, GM-CSF, ICAM-1, IFN-γ, IL-1α, IL1β, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8,
IL-8 (HA*), IL-10, IL-12p70, IL-12/IL-23p40, IL-13, IL-15, IL-16, IL-17A, IP-10, MCP-1, MCP-4, MDC, MIP-1α,
MIP-1β, PIGF, SAA, TARC, Tie-2, TNF-α, TNF-β, VCAM-1, VEGF-A, VEGF-C, VEGF-D |
| Cytokine 44-Plex Kit (K15249G) |
Eotaxin, Eotaxin-3, GM-CSF, IFN-γ, IL-1α, IL-1β, IL-1RA, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8,
IL-8 (high abundance), IL-9, IL-12p70, IL-12/IL-23p40, IL-13, IL-16, IL-17A, IL-17 A/F, IL-17B, IL-10, IL-15,
IL-17C, IL-17D, IL-21, IL-22, IL-23, IL-27, IL-31, IP-10, MCP-1, MCP-4, MDC, MIP-1α, MIP-1β, MIP-3α, TARC,
TNF-α, TNF-β, TSLP, VEGF-A |
Cytokine 30-Plex Kit
(K15054G) |
Eotaxin, Eotaxin-3, GM-CSF, IFN-γ, IL-1α, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-8 (HA*), IL-10,
IL-12p70, IL-12/IL-23p40, IL-13, IL-15, IL-16, IL-17A, IP-10, MCP-1, MCP-4, MDC, MIP-1α, MIP-1β, TARC,
TNF-α, TNF-β, VEGF-A |
Proinflammatory Panel 1
(K15049G) |
IFN-γ, IL-1β, IL-2, IL-4, IL-6, IL-8, IL-10, IL-12p70, IL-13, TNF-α |
| TH17 Panel 1 (K15085G) |
IL-17A (Version B), IL-21, IL-22, IL-23, IL-27, IL-31, MIP-3α |
| Cytokine Panel 1 (K15050G) |
GM-CSF, IL-1α, IL-5, IL-7, IL-12/IL-23p40, IL-15, IL-16, IL-17A, TNF-β, VEGF-A |
| Cytokine Panel 2 (K15084G) |
IL-17A/F, IL-17B, IL-17C, IL-17D, IL-1RA, IL-3, IL-9, TSLP |
Chemokine Panel 1
(K15047G) |
Eotaxin, Eotaxin-3, IL-8 (HA*), IP-10, MCP-1, MCP-4, MDC, MIP-1α, MIP-1β, TARC |
| Angiogenesis Panel 1 (K15190G) |
FGF (basic), Flt-1, PIGF, Tie-2, VEGF-A**, VEGF-C, VEGF-D |
| Vascular Injury Panel 2 (K15198G) |
CRP, ICAM-1, SAA, VCAM-1 |
Neuroinflammation Panel 1
(K15210G) |
CRP, Eotaxin, Eotaxin-3, Flt-1/VEGFR-1, ICAM-1, IFN-γ, IL-1α, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10,
IL-12/IL-23p40, IL-13, IL-15, IL-16, IL-17A, IP-10, MCP-1, MCP-4, MDC, MIP-1α, MIP-1β, PlGF, SAA, TARC,
Tie-2, TNF-α, TNF-β, VCAM-1, VEGF-A, VEGF-C, VEGF-D |
Aβ Peptide Panel 1 (6E10)
(K15200G) |
Aβ38 (6E10), Aβ40 (6E10), Aβ42 (6E10) |
Aβ Peptide Panel 1 (4G8)
(K15199G) |
Aβ38 (4G8), Aβ40 (4G8), Aβ42 (4G8) This product is suitable for human, mouse, and rat samples. |
| Metabolic Panel 1 (K15325DG) |
C-Peptide, GIP (active), GLP-1 (active), Glucagon, Insulin, Leptin, and PP |
| Viral Panel 1 Kit (K15345D) |
IL-1β, IL-6, IL-8, TNF-α |
| Viral Panel 2 Kit (K15346D) |
IFN-γ, IL-1β, IL-4, IL-6, IL-8, IL-10, TNF-α |
| Viral Panel 3 Kit (K15347D) |
IFN-γ, IL-1β, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12p70, TNF-α |
| NHP |
Cytokine 24-Plex Kit
(K15058G) |
Eotaxin-3, GM-CSF, IFN-γ, IL-1β, IL-2, IL-5, IL-6, IL-7, IL-8, IL-8 (HA*), IL-10, IL-12/IL-23p40, IL-15, IL-16,
IL-17A, IP-10, MCP-1, MCP-4, MDC, MIP-1α, MIP-1β, TARC, TNF-β, VEGF-A |
| Cytokine Panel 1 (K15057G) |
GM-CSF, IL-5, IL-7, IL-12/IL-23p40, IL-15, IL-16, IL-17A, TNF-β, VEGF-A |
| Proinflammatory Panel 1 (K15056G) |
IFN-γ, IL-1β, IL-2, IL-6, IL-8, IL-10 |
| Chemokine Panel 1 (K15055G) |
Eotaxin-3, IL-8 (HA*), IP-10, MCP-1, MCP-4, MDC, MIP-1α, MIP-1β, TARC |
| Metabolic Panel 1 (K15332G) |
C-Peptide, GIP (active), GLP-1 (active), Glucagon, Insulin, and PP |
| Viral Panel 1 Kit (K15348D) |
IL-1β, IL-6, IL-8 |
| Viral Panel 2 Kit (K15349D) |
IFN-γ, IL-1β, IL-6, IL-8, IL-10 |
| Viral Panel 3 Kit (K15350D) |
IFN-γ, IL-1β, IL-5, IL-6, IL-8, IL-10 |
| Mouse |
Proinflammatory Panel 1 (K15048G) |
IFN-γ, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-10, IL-12p70, KC/GRO, TNF-α |
| Cytokine Panel 1 (K15245G) |
IL-9, IL-15, IL-17A/F, IL-27p28/IL-30, IL-33, IP-10, MIP-1α, MIP-2, MCP-1 |
| Cytokine 19-Plex Kit (K15255G) |
IFN-γ, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12p70, IL-15, IL-17 A/F, IL-27p28/IL-30, IL-33, IP-10, KC/GRO, MCP-1,
MIP-1α, MIP-2, TNF-α |
| Metabolic Panel 1 (K15333G) |
GLP-1 (active) and Glucagon |
| Rat |
Metabolic Panel 1 (K15334G) |
GIP (active), GLP-1 (active), and Glucagon |
| Proinflammatory Panel 2 (K15059G) |
IFN-γ, IL-1β, IL-4, IL-5, IL-6, IL-10, IL-13, KC/GRO, TNF-α |
| Canine |
Metabolic Panel 1 (K15335G) |
GIP (active), GLP-1 (active), Glucagon, and Insulin |
|
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