Recombinant Human ST3Gal1 (CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,3-sialyltransferase 1), soluble fragment



ST3Gal1 catalyzes transfer of the sialic acid Neu5Ac from CMP-Neu5Ac (cytidine 5'-monophosphono-N-acetylneuraminic acid) to Galβ1,3GalNAc on glycoproteins or glycolipids in an α2,3 linkage (1).  It is a type II transmembrane protein that is normally localized to the golgi, but can be cleaved to yield a soluble product.  Sialylation of the core-1 structure (Galβ1,3GalNAc-O-Ser/Thr) by ST3Gal1 prevents formation of the core-2 structure (GlcNAcβ1,6(Galβ1,3)GalNAc-Ser/Thr).  On CD8+ T-cells this prevents induction of either apoptosis or differentiation into memory cells (2).  Overexpression of ST3Gal1 in breast cancer cells correlated with the presence of the core-1 based marker SM3, whereas healthy cells displayed a higher ratio of core-2 based epitopes (3).  Overexpression of ST3Gal1 in colorectal carcinoma was found to be related to poor patient survival (4).

  1. Kitagawa, H. & Paulson, J.C. (1994) J. Biol. Chem. 269:17872.
  2. Priatel, J.J.  et al. (2000) Immunity 12:273.
  3. Dalziel, M. et al. (2001) J. Biol. Chem. 276:11007.
  4. Schneider, F. et al. (2001) Cancer Res. 61:4605.

Catalog #:  SBH-003- ST3Gal1

Source :  Insect Cells, Accession # Q11201

Formulation:  Sterile filtered solution in 25mM Tris pH7.5 and 150mM NaCl, at a stock concentration of 100 ug/mL.

Stability:  Stable for 4 weeks at 4C.  Stable for 6 months at -80C.  Avoid repeated freeze-thaw cycles.

Purity:  Greater than 90% by SDS-PAGE.

Reconstitution:  N/A

Biological Activity:  In progress.

PubMed  Link: ST3Gal1 Human

Usage:  For research only. Not for use in diagnostic or therapeutic procedures.

Country of Origin:  USA


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